yxeQ [2018-03-05 09:27:37]

unknown, the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant

locus: BSU39460

pI: 5.94

mw: 47.64 kDa

protein length: 445 aa

gene length: 1335 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 4,055,536 → 4,056,873

The protein

Protein family

• prpD family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

  regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B713 (yxeQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1712 NBRP B. subtilis, Japan]
• BKE39460 (Δ[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGCTGTCAGCCCTTGTT, downstream forward: _UP4_TAAGATCAACTCTATTCGGT
• BKK39460 (Δ[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGCTGTCAGCCCTTGTT, downstream forward: _UP4_TAAGATCAACTCTATTCGGT

References

• 16513748